Pseudomonas putida DNA replication and repair protein recF (recF) -E. coli
Recombinant Pseudomonas putida DNA replication and repair protein recF (recF)
recombination protein F; DNA replication and repair protein RecF; recombination protein F;
recF; recF; PputW619_0003
DNA replication and repair protein recF(recF) is a recombinant protein expressed in E. coli. The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.
E Coli ; Yeast ; Baculovirus ; Mammalian Cell
Greater than 90% (determined by SDS-PAGE)
This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.
This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.
Pseudomonas is a Gram-negative bacteria. Pseudomonas genomic sequences are used in medical research. A problem in hostpital is often the Pseudomonas Aeruginosa containing multi drug resistancy properties that are transmitted through plasmids. The MDR property is transmitter by the R plasmid.
Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.