Basic information

Name

Enterobacteria phage M13 DNA-Binding protein G5P (V) -E. coli

Price

1332 EUR

Size

1000ug

Catalog no

GEN1106512.E.coli

Extended information

Long name

Recombinant Enterobacteria phage M13 DNA-Binding protein G5P (V)

Alternative names

helix destabilising protein; helix destabilising protein;

Gene name

N/A

Other gene names

V;

General description

DNA-Binding protein G5P (V) is a recombinant protein expressed in E. coli. The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.

Product category

Recombinant Proteins

Expression system

E. coli

Available also expressed in:

E Coli ; Yeast ; Baculovirus ; Mammalian Cell

Purity

Greater than 90% (determined by SDS-PAGE)

Form

Lyophilized protein

Storage

This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.

Applications

This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.

Gene

M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.