Basic information

Name

Salmonella newport DNA replication and repair protein recF (recF) -E. coli

Price

2017 EUR

Size

1000ug

Catalog no

GEN1156572.E.coli

Extended information

Long name

Recombinant Salmonella newport DNA replication and repair protein recF (recF)

Alternative names

recombination protein F; DNA replication and repair protein RecF;

Gene name

recF

Other gene names

recF;

General description

DNA replication and repair protein recF(recF) is a recombinant protein expressed in E. coli. The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.

Product category

Recombinant Proteins

Expression system

E. coli

Available also expressed in:

E Coli ; Yeast ; Baculovirus ; Mammalian Cell

Purity

Greater than 90% (determined by SDS-PAGE)

Form

Lyophilized protein

Storage

This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.

Applications

This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.

Disease

Salmonella typhimurium, enteriditis and Salmonella paratyphi antibodies or media detect this rod-shaped (bacillus) gram-negative bacteria of the Enterobacteriaceae family. The two species of Salmonella are Salmonella enterica and Salmonella bongori. Salmonella enterica is the type species and is further divided into six subspecies that include over 2500 serovars. S. enterica subspecies are found worldwide in all warm-blooded animals, and in the environment. S. bongori is restricted to cold-blooded animals particularly reptiles. Strains of Salmonella cause illnesses such as typhoid fever, paratyphoid fever, and food poisoning (salmonellosis).

Test

Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.