Basic information

Name

Faba bean necrotic yellows virus Master replication protein (DNA-R) -Mammalian Cell

Price

2348 EUR

Size

100ug

Catalog no

GEN1217962.Mammalian Cell

Extended information

Long name

Recombinant Faba bean necrotic yellows virus Master replication protein (DNA-R)

Alternative names

Master replication protein; Master replication protein; ATP-dependent helicase C2; Replication-associated protein 2; Rep2

Gene name

DNA-R

Other gene names

DNA-R; C2; M-Rep; Rep2

General description

Master replication protein (DNA-R) is a recombinant protein expressed in Mammalian Cell . The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.

Product category

Recombinant Proteins

Expression system

Mammalian Cell

Available also expressed in:

E Coli ; Yeast ; Baculovirus ; Mammalian Cell

Purity

Greater than 90% (determined by SDS-PAGE)

Form

Lyophilized protein

Storage

This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.

Applications

This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.

Description

For cells, cell lines and tissues in culture till half confluency.

Test

Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.

Properties

Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.